{"id":23718,"date":"2023-03-06T13:40:53","date_gmt":"2023-03-06T12:40:53","guid":{"rendered":"https:\/\/www.dexeuscampus.com\/blog\/uncategorized\/impact-of-embryo-biopsy-technique-on-the-prevalence-of-mosaicism\/"},"modified":"2023-03-06T16:37:14","modified_gmt":"2023-03-06T15:37:14","slug":"impact-embryo-biopsy-mosaicism","status":"publish","type":"post","link":"https:\/\/www.dexeuscampus.com\/en\/blog\/assisted-reproduction\/impact-embryo-biopsy-mosaicism\/","title":{"rendered":"Impact of embryo biopsy technique on the prevalence of mosaicism"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"23718\" class=\"elementor elementor-23718 elementor-23534\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b292dd9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b292dd9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9d0385f\" data-id=\"9d0385f\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-002f025 elementor-widget elementor-widget-heading\" data-id=\"002f025\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">The method used for trophectoderm biopsy does not affect the incidence of mosaicism<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f686d39 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f686d39\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e4cf805\" data-id=\"e4cf805\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-424566a elementor-widget elementor-widget-text-editor\" data-id=\"424566a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>A team from the Reproductive Medicine Department at Dexeus Mujer, led by the embryologist Lluc Coll, carried out a prospective observational study to determine whether the prevalence of mosaic embryos in ART cycles with preimplantation genetic testing for aneuploidy (PGT-A) is associated with the trophectoderm biopsy methodology used. Previous research suggested that the biopsy technique could lead to artefactual results compatible with mosaicism. The study by Dexeus Mujer was conducted in a single IVF-PGT-A setting, between May 2019 and May 2021. Trophectoderm biopsies were analysed by mass sequencing.<\/p>\n<p>Mosaicism was analysed in relation to biopsy methodology (number of laser pulses applied and <em>pulling<\/em> vs <em>flicking<\/em>), time from biopsy to sample <em>tubing<\/em> (min), and time of sample cryopreservation until amplification (days). As a secondary objective, the correlation between the biopsy methodology and the clinical outcome of transferred euploid blastocysts was also studied.<\/p>\n<p>The results revealed that none of the variables analysed is associated with the prevalence of mosaicism. Therefore, the authors concluded that, as long as biopsies are performed following standardised, high-quality procedures, no specific approach increases the incidence of artefactual mosaicism. Likewise, the clinical outcomes obtained after euploid embryo transfers were unrelated to the biopsy methodology.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5716bfc elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5716bfc\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-417701c\" data-id=\"417701c\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b35c714 elementor-widget elementor-widget-image\" data-id=\"b35c714\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t<figure class=\"wp-caption\">\n\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"350\" height=\"370\" src=\"https:\/\/www.dexeuscampus.com\/wp-content\/uploads\/2023\/03\/zoomPro2023-01-1a.png\" class=\"attachment-large size-large wp-image-23795\" alt=\"Impacto de la t\u00e9cnica de biopsia embrionaria en la prevalencia de mosaicismo\" srcset=\"https:\/\/www.dexeuscampus.com\/wp-content\/uploads\/2023\/03\/zoomPro2023-01-1a.png 350w, https:\/\/www.dexeuscampus.com\/wp-content\/uploads\/2023\/03\/zoomPro2023-01-1a-284x300.png 284w\" sizes=\"(max-width: 350px) 100vw, 350px\" \/>\t\t\t\t\t\t\t\t\t\t\t<figcaption class=\"widget-image-caption wp-caption-text\">Mosaic blastocyst scheme<\/figcaption>\n\t\t\t\t\t\t\t\t\t\t<\/figure>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2287fe9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2287fe9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6cde540\" data-id=\"6cde540\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e9f3614 elementor-widget elementor-widget-text-editor\" data-id=\"e9f3614\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Reference study:<\/strong><br \/><a style=\"font-size: 16px; font-style: italic; color: #4f91cd; text-decoration: underline;\" title=\"The effect of trophectoderm biopsy technique and sample handling on artefactual mosaicism\" href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35294709\/\" target=\"_blank\" rel=\"noopener\">The effect of trophectoderm biopsy technique and sample handling on artefactual mosaicism<\/a><br \/><span style=\"font-size: 16px; font-style: italic;\">L. Coll, M. Parriego, B. Carrasco, I. Rodr\u00edguez, M. Boada, B. Coroleu, N.P Polyzos, F. Vidal, A. Veiga<\/span><br \/><span style=\"font-size: 16px; font-style: italic;\">Assist Reprod Genet. 2022 Jun;39(6):1333-1340. doi: 10.1007\/s10815-022-02453-9.<br \/>Epub 2022 Mar 16.<\/span><\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>An observational study led by the embryologist Lluc Coll analysed whether the prevalence of mosaic embryos in ART cycles with preimplantation genetic testing for aneuploidy (PGT-A) is associated with the trophectoderm biopsy technique used or with the time elapsed until the tubing of the sample.<\/p>\n","protected":false},"author":3,"featured_media":23537,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[205],"tags":[],"class_list":["post-23718","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-assisted-reproduction"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - 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