Impact of embryo biopsy technique on the prevalence of mosaicism

Impacto de la técnica de biopsia embrionaria en la prevalencia de mosaicismo

Impact of embryo biopsy technique on the prevalence of mosaicism

The method used for trophectoderm biopsy does not affect the incidence of mosaicism

A team from the Reproductive Medicine Department at Dexeus Mujer, led by the embryologist Lluc Coll, carried out a prospective observational study to determine whether the prevalence of mosaic embryos in ART cycles with preimplantation genetic testing for aneuploidy (PGT-A) is associated with the trophectoderm biopsy methodology used. Previous research suggested that the biopsy technique could lead to artefactual results compatible with mosaicism. The study by Dexeus Mujer was conducted in a single IVF-PGT-A setting, between May 2019 and May 2021. Trophectoderm biopsies were analysed by mass sequencing.

Mosaicism was analysed in relation to biopsy methodology (number of laser pulses applied and pulling vs flicking), time from biopsy to sample tubing (min), and time of sample cryopreservation until amplification (days). As a secondary objective, the correlation between the biopsy methodology and the clinical outcome of transferred euploid blastocysts was also studied.

The results revealed that none of the variables analysed is associated with the prevalence of mosaicism. Therefore, the authors concluded that, as long as biopsies are performed following standardised, high-quality procedures, no specific approach increases the incidence of artefactual mosaicism. Likewise, the clinical outcomes obtained after euploid embryo transfers were unrelated to the biopsy methodology.

Impacto de la técnica de biopsia embrionaria en la prevalencia de mosaicismo
Mosaic blastocyst scheme

Reference study:
The effect of trophectoderm biopsy technique and sample handling on artefactual mosaicism
L. Coll, M. Parriego, B. Carrasco, I. Rodríguez, M. Boada, B. Coroleu, N.P Polyzos, F. Vidal, A. Veiga
Assist Reprod Genet. 2022 Jun;39(6):1333-1340. doi: 10.1007/s10815-022-02453-9.
Epub 2022 Mar 16.

¿Cómo podemos ayudarte?

Te informamos que los datos de carácter personal que proporciones rellenando el presente formulario serán tratados por Fundación Dexeus Mujer como responsable de esta web. La finalidad de la recogida y tratamiento de estos datos es contactar contigo en referencia a tu solicitud y en caso de que expresamente nos autorices enviarte información sobre actividades, promociones y/o servicios y nuestro boletín informativo periódico. La legitimación se realiza a través del consentimiento del interesado. Podrás ejercer tus derechos de acceso, rectificación, limitación y suprimir los datos ante el delegado de protección de datos en así como el derecho a presentar una reclamación ante una autoridad de control. Puedes consultar la información adicional y detallada sobre Protección de Datos en nuestra política de privacidad.

How can we help you?

We inform you that the personal data that you provide by filling in this form will be treated by Dexeus Mujer Foundation as responsible for this website. The purpose of collecting and processing this data is to contact you regarding your request and in the event that you expressly authorize us to send you information on activities, promotions and / or services and our periodic newsletter. The legitimation is carried out through the consent of the interested party. You can exercise your rights of access, rectification, limitation and delete the data before the data protection officer at as well as the right to file a claim with a control authority. You can consult the additional and detailed information on Data Protection in our privacy policy.