The method used for trophectoderm biopsy does not affect the incidence of mosaicism
A team from the Reproductive Medicine Department at Dexeus Mujer, led by the embryologist Lluc Coll, carried out a prospective observational study to determine whether the prevalence of mosaic embryos in ART cycles with preimplantation genetic testing for aneuploidy (PGT-A) is associated with the trophectoderm biopsy methodology used. Previous research suggested that the biopsy technique could lead to artefactual results compatible with mosaicism. The study by Dexeus Mujer was conducted in a single IVF-PGT-A setting, between May 2019 and May 2021. Trophectoderm biopsies were analysed by mass sequencing.
Mosaicism was analysed in relation to biopsy methodology (number of laser pulses applied and pulling vs flicking), time from biopsy to sample tubing (min), and time of sample cryopreservation until amplification (days). As a secondary objective, the correlation between the biopsy methodology and the clinical outcome of transferred euploid blastocysts was also studied.
The results revealed that none of the variables analysed is associated with the prevalence of mosaicism. Therefore, the authors concluded that, as long as biopsies are performed following standardised, high-quality procedures, no specific approach increases the incidence of artefactual mosaicism. Likewise, the clinical outcomes obtained after euploid embryo transfers were unrelated to the biopsy methodology.
The effect of trophectoderm biopsy technique and sample handling on artefactual mosaicism
L. Coll, M. Parriego, B. Carrasco, I. Rodríguez, M. Boada, B. Coroleu, N.P Polyzos, F. Vidal, A. Veiga
Assist Reprod Genet. 2022 Jun;39(6):1333-1340. doi: 10.1007/s10815-022-02453-9.
Epub 2022 Mar 16.